Major Instrumentation

  • Hamilton Starlet Liquid Handler

  • Perkin Elmer Sciclone ALH3000 Liquid Handler

  • V&P Scientific 96 Pin-Tool

  • V&P Scientific 384 Pin-Tool

  • HP D300 Digital Dispenser

  • Thermo CellInsight NXT High Content Imager

  • BioTek Synergy 4 Microplate Reader

 

HTSSL Resources

The OSU College of Pharmacy’s High-Throughput Screening Services Laboratory (HTSSL) is a fee-for-service facility with instrumentation and chemical libraries for small molecule screening, bioactivity characterization, and automation services. HTSSL occupies over 1,700 square feet of BSL-2 certified lab space in rooms 123 and 125 of the Pharmacy Building. The facility has robotics equipment for automation of liquid handling and assay readouts. The readout instrumentation includes microplate readers for luminescence, fluorescence, FRET, fluorescence polarization, and absorbance assays. Additionally, the Thermo CellInsight NXT high-content imager is available for multi-parameter image analysis of individual or populations of cells. Please see equipment for full listing. The Dotmatics Enterprise Data Management software suite is used for compound management, data analysis, analysis workflows, data visualization, and the electronic laboratory notebook. HTSSL has multiple user models that include full-service, client-assisted, and client-run screening. HTSSL staff is on-site and available for training, troubleshooting, and conducting screens as needed. Services are available to both public and private researchers.

 

Full Equipment List

BSL-2 compliant laboratory space with Class II Biological Safety Cabinets
AMS Chemical Fume Hood
Temperature-controlled CO2 incubators (2)
Temperature-controlled standard lab incubator
Inverted light microscopes (2)
Olympus IX51 fluorescence microscope
4°C refrigerators, -20°C freezers (10), -80°C ultra-low freezers, liquid nitrogen storage dewar
Autoclave sterilizers
Thermo Sorvall benchtop refrigerated centrifuge (ST-40R)
Bechman Coulter benchtop refrigerated centrifuge (Allegra X-15R)
Thermo RT1 benchtop centrifuge
Orbital incubator shaker (floor model New Brunswick Excella E24)
Invitrogen Countess® automated cell counter
Rainin Liquidator 96-well manual pipetting manifold
Biotek ELx405 CW microplate washers (2) (each with integrated BioStack microplate stacker)
Mettler Toledo balance (AB135-S Precision Balance)
PlateLoc Velocity II microwell plate sealer (2)
Liquid Handling Robotic Workstations (2)
Perkin Elmer STACCATO Integrated High Throughput Screening System
Sciclone ALH3000
Sciclone 96-well mandrel
Sciclone 384-well mandrel
Sciclone Z-8 liquid handler
Integrated barcode reader
V&P Scientific 96 pin replicator
V&P Scientific 384 pin replicator
Twister II microplate handlers
BioTek Synergy 4 microplate reader
Cytomat 2C live-cell incubator
Hamilton Microlab Starlet
CO-RE 96-channel pipetting head
8 independent pipetting channels with gripper attachments
iSWAP gripper
Perkin Elmer Plate Stack (2)
Thermo CellInsight NXT High Content Screening Platform
Automated inverted microscope
High-resolution, high-sensitivity, CCD camera for fully auto-focus imaging of wells from microplates
Four LED-based excitation and detection channels
Three microscope objectives: 4X, 10X, and 20X, air lenses
Perkin Elmer Plate Handler II 6-axis robotic arm
Compact Liquid Handling Robotic Workstation:  BioTek Precision X2 microplate sampler
Stand-Alone Microplate Reader:  Turner Biosystems Modulus microplate reader
HP D300 Digital Titration Instrument (2)
Matrix WellMate microplate fluid dispensers (2) (one stand alone and one with integrated plate stacker)
BioTek Multiflo FX fluid dispenser