Mark Leid, Ph.D.

Professor
Assistant Dean for Scholarship
Department of Pharmaceutical Sciences

Contact Information

College of Pharmacy
Oregon State University
203 Pharmacy Building
Corvallis, OR 97331-3507
Phone: 541-737-5809
Mark.Leid@oregonstate.edu
Leid's page at the OSU Center for Gene Research and Biotechnology

Education

Institut de Chimie Biologique, Strasbourg, France, Postdoctoral Fellow, 1989-1992 Oregon State University, Ph.D., 1989 Washington State University, B.Pharm., 1983

Research

The focus of Mark Leid’s laboratory is the mechanism of signal transduction mediated by members of the nuclear receptor superfamily, a large group of ligand-dependent transcription factors. He uses a variety of genetic, molecular biological and biochemical techniques to dissect the molecular basis of ligand activation of nuclear receptors and the mechanisms underlying the ability of these proteins to modulate transcriptional processes, the latter of which involves interaction of the receptors with two other groups of cellular proteins known as transcriptional corepressors and coactivators. In addition, he is interested in the isolation of genes that are transcriptional targets of nuclear receptors toward the goal of understanding the cellular and organismal effects of nuclear receptor activation. Two subfamilies of nuclear receptors are currently studied in the laboratory: (1) the retinoic acid (RAR) and retinoid X (RXR) receptors that mediate cellular effects of vitamin A (retinoic acids) and related retinoids, (2) the COUP-TF family of orphan receptors that appear to function as constitutive repressors of transcription through interaction with the CTIP proteins cloned in this laboratory.

Selected Publications

Avram D, Fields A, Senawong T, Topark-Ngarm A, Nevrivy DJ, Ishmael JE, Leid M. COUP-TF-interacting Protein 1 (CTIP1) is a Sequence-Specific DNA Binding Protein. Biochemical Journal. In press.

Leid M. Retinoids. In: Abraham D, ed. Burger's Textbook of Medicinal Chemistry and Drug Discovery, 6th ed. New York: Wiley & Sons. In press.

Zhang Y, Mohammad R, Rishi AK, Farhana L, Dawson MI, Feng K-C, Leid M, Peterson VJ, Zhang X-k, Edelstein M, Eilander D, Biggar S, Wall N, Reichert U, Fontana JA. Induction of apoptosis of human B-CLL and ALL cells by a novel retinoid and its non-retinoidal analog. Blood. In press.

Ruby CE, Leid M, Kerkvliet NI. 2,3,7,8-Tetrachlorodibenzo-p-dioxin suppresses tumor necrosis factor-alpha and anti-CD40-induced activation of NF-kappa B/Rel in dendritic cells: p50 homodimer activation is not affected. Molecular Pharmacology. 2002;62:722-728.

Peterson VJ, Barofsky E, Deinzer ML, Dawson MI, Feng K-c, Zhang X-k, Madduru MR, Leid M. Mass spectrometric analysis of agonist-induced retinoic acid receptor g conformational change. Biochemical Journal. 2002;362:173-81.

Dawson MI, Hobbs PD, Peterson VJ, Leid M, Lange C, Chen G-q, Gu J, Kolluri SK, Zhang X-k, Zhang Y, Fontana JA. Induction of apoptosis in cancer cells by a novel analog of 6-[3-(1-Adamantyl)-4-hydroxyyphenyl]-2-naphthalenecarboxylic acid (AHPN) lacking retinoid receptor tranascriptional activation activity. Cancer Research. 2001;61:4723-4730.

Soderstrom K, Leid M, Moore FL, Murray TF. Behavioral, pharmacological and molecular characterization of an amphibian cannabinoid receptor. Journal of Neurochemistry. 2000;75:413-423.

Fontana JA, Dawson MI, Leid M, Rishi AK, Zhang Y, Hsu CA, Lu JS, Peterson VJ, Cao W-R, Shroot B, Reichert U. Identification of a unique binding protein specific for a novel retinoid inducing cellular apoptosis. International Journal of Cancer. 2000;86:474-479.

Avram D, Fields A, Pretty On Top K, Nevrivy DJ, Lee S-H, Ishmael JE, Leid M. Isolation of a novel family of C2H2 zinc finger proteins implicated in transcriptional repression mediated by chicken ovalbumin upstream promoter transcription factor (COUP-TF) orphan nuclear receptors. Journal of Biological Chemistry. 2000;275:10315-10322.

Nevrivy DJ, Peterson VJ, Avram D, Ishmael JE, Hansen SG, Dowell P, Hruby DE, Dawson MI, Leid M. Interaction of GRASP, a protein encoded by a novel retinoic acid-induced gene, with members of the cytohesin family of guanine nucleotide exchange factors. Journal of Biological Chemistry. 2000;275:16827-16836.

Avram D, Ishmael JE, Nevrivy DJ, Lee S-H, Dowell P, Leid M. Heterodimeric interactions between COUP-TF family members ARP1 and Ear2. Journal of Biological Chemistry. 1999;274:14331-14336.

Dowell P, Ishmael JE, Avram D, Nevrivy DJ, Leid M. Identification of nuclear receptor corepressor as a peroxisome proliferator-activated receptor a interacting protein. Journal of Biological Chemistry. 1999;274:15901-15907.

Schimerlik MI, Peterson VJ, Hobbs PD, Dawson MI, Leid M. Kinetic and thermodynamic analysis of 9-cis-retinoic acid binding to Retinoid X Receptor a. Biochemistry. 1999;38:6732-6740.

Bouvier C, Avram D, Peterson VJ, Hettinger-Smith B, Soderstrom K, Murray TF, Leid M. Noradrenergic CATH.a cells express predominantly delta opioid receptors. European Journal of Pharmacology. 1998;348:85-93.

Dowell P, Peterson VJ, Zabriskie TM, Leid M. Ligand-induced peroxisome proliferator-activated receptor alpha conformational change. Journal of Biological Chemistry. 1997;272:2013-2020.

Dowell P, Ishmael JE, Avram D, Peterson VJ, Nevrivy DJ, Leid M. p300 functions as a coactivator for the peroxisome proliferator-activated receptor a. Journal of Biological Chemistry. 1997;272:33435-33443.

Kastner P, Mark M, Leid M, Gansmuller A, Grondona JM, Décimo D, Krezzel W, Dierich A, Chambon P. Abnormal spermatogenesis in RXRbeta mutant mice. Genes & Development. 1996;10:80-92.

Ishmael JE, Franklin PH, Murray TF, Leid M. High level expression of the NMDAR1 glutamate receptor subunit in electroporated COS cells. Journal of Neurochemistry. 1996;67:1500-1510.

Leid M. Ligand-induced alteration of the protease sensitivity of retinoid X receptor alpha. Journal of Biological Chemistry. 1994;269:14175-14181.

Zhang Y, Mohammad R, Rishi AK, Farhana L, Dawson MI, Feng K-C, Leid M, Peterson VJ, Zhang X-k, Edelstein M, Eilander D, Biggar S, Wall N, Reichert U, Fontana JA . Induction of apoptosis of human B-CLL and ALL cells by a novel retinoid and its non-retinoidal analog. Blood. In press.