Ryszard Zielke

Assistant Professor (Sr Res)
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Office: 541-737-5381

Weniger Hall

Weniger Hall 433

103 SW Memorial Place

103 SW Memorial Place
Corvallis, OR 97331
Credentials: 
University of Michigan, Postdoctoral Fellow, 2006-2011 University of Gdansk, PhD (Molecular Biology), 2006 University of Gdansk, M.Sc (Microbiology), 2001

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At OSU
Affiliated with: 
Pharmacy Professnl Instr
Faculty Type: 
Pharmaceutical Sciences
Research/Career Interests: 

The emergence and increasing occurrence of bacterial strains that are resistant to all classes of available antibiotics is a global problem. Current antimicrobials target a relatively small number of essential gene functions including: inhibition of cell wall biosynthesis, and synthesis of macromolecules (proteins, DNA and RNA). Treatment of infections caused by antibiotic resistant bacteria requires new approaches and agents with novel modes of action. The bacterial extracellular proteome (cell envelope, membrane vesicles and secreted proteins) plays a fundamental role in establishing infection by enabling the microbes to adhere to and invade host cells, facilitating nutrient acquisition, host tissue destruction, and suppression of host immune responses. Hence the components of the extracellular proteome are promising targets for drugs/vaccines aimed at preventing bacterial infections. The long-term goal of our research is to enhance our understanding of the phenotypic plasticity of the bacterial extracellular proteome and utilize this information to identify attractive targets for development of new therapeutic interventions. Currently, our research focuses on the role of bacterial extracellular proteomes in colonization and circumvention or exploitation of host immune response using two model organisms Vibrio cholerae and Neisseria gonorrhoeae. We examine these issues using comprehensive proteomic studies, chemical genomics, and state of the art genetic, molecular and biochemical methods.

Selected Publications

Sikora, AE, Zielke, RA, Lawrence, DA, Andrews, PC, Sandkvist M (2011). Proteomic analysis of the Vibrio cholerae type II secretome reveals new proteins including three related serine proteases. J. Biol. Chem. 286(19):16555-66.

Jomaa A, Stewart G, Martín-Benito J, Zielke R, Campbell TL, Maddock JR, Brown ED, Ortega J. (2011).  Understanding ribosome assembly: the structure of in vivo assembled immature 30S subunits revealed by cryo-electron microscopy. RNA 17(4):697-709.

Jagtap P, Michailidis G, Zielke R, Walker AK, Patel N, Strahler JR, Driks A, Andrews PC, Maddock JR. (2006).  Early events ofBacillus anthracis germination identified by time-course quantitative proteomics. Proteomics. 6(19):5199-211.

Sikora AE, Zielke R, Datta K, Maddock JR. (2006). The Vibrio harveyi GTPase CgtAV is essential and is associated with the 50S ribosomal subunit. J. Bacteriol. 188(3):1205-10. 

Zielke R, Sikora A, Dutkiewicz R, Wegrzyn G, Czyz A. (2003). Involvement of the  cgtA gene function in stimulation of DNA repair inEscherichia coli and Vibrio harveyi. Microbiology. 149(Pt 7):1763-70.  

History

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