The research in Xihou Yin’s laboratory is focused on the natural product antibiotic biosynthesis, regulation and resistance from representatives of a variety of bacterial genera including Streptomyces, Actinoplanes and Paenibacillus. We employ combined techniques and approaches from molecular genetics, molecular biology, microbiology, biochemistry, natural product chemistry and modern instrument analysis to address all aspects of antibiotic production. Below are the representative areas of my longstanding interests.
1) Functional characterization of antibiotic biosynthetic gene clusters.
2) Genetic engineering and reprogramming of nonribosomal peptide antibiotic biosynthetic pathways toward novel analogs with improved properties.
3) Self-resistance mechanisms of peptide antibiotics in Actinomyces.
4) Genetic regulation of antibiotic production and strain improvement.
5) Genomic and proteomic approaches to antibiotic regulatory networks and resistant components, and the activation of cryptic antibiotic biosynthesis pathways.
6) Design, development and optimization of the vector-host systems to facilitate the functional analysis of biosynthetic genes and gene clusters.
7) Biological evaluation of the antimicrobial and immunomodulatory activities of natural products from bacterial and fungal origins.
Li S, Yang D, Qiu M, Shao J, Guo R, Shen B, Yin X, Zhang R, Zhang N, Shen Q. Complete genome sequence of Paenibacillus polymyxa SQR-21, a plant growth-promoting rhizobacterium with antifungal activity and rhizosphere colonization ability. Genome Announc. 2014; 2(2): e00281-14
Li S, Zhang R, Wang Y, Zhang N, Shao J, Qiu M, Shen B, Yin X, Shen Q. Promoter analysis and transcription regulation of fus gene cluster responsible for fusaricidin synthesis of Paenibacillus polymyxa SQR-21. Appl Microbiol Biotechnol. 2013; 97(21):9479-9489.
Wang, Y.; Weng, J.; Waseem, R.; Yin, X.; Zhang, R.; Shen, Q. Bacillus subtilis genome editing using ssDNA with short homology regions. Nucleic Acids Res. 2012.1-9. doi: 10.1093/nar/gks.
Wang, Y.; Chen, Y.; Shen, Q. and Yin, X. Molecular cloning and identification of the laspartomycin biosynthetic gene cluster fromStreptomyces viridochromogenes. Gene 2011. 483 (1-2):11-21.
Nie, M.; Yin, X.; Jia, J.; Wang, Y.; Liu, S.; Shen, Q.; Li, P.and Wang, Z. Production of a novel bioflocculant MNXY1 by Klebsiella pneumoniae strain NY1 and application in precipitation of cyanobacteria and municipal wastewater treatment. J. Appl. Microbiol. 2011.111(3):547-58.
Yin, X.; Chen, Y.; Zhang, L.; Wang, Y.; Zabriskie, T.M. Enduracidin Analogues with Altered Halogenation Patterns Produced by Genetically Engineered Strains of Streptomyces fungicidicus. J. Nat. Prod. 2010. 7:583-589.
Nie, M.; Yin, X.; Ren, C.; Wang, Y.; Xu, F.; Shen, Q.; Liu, S. Novel Rhamnolipid Biosurfactants Produced by a Polycyclic Aromatic Hydrocarbon-Degrading Bacterium Pseudomonas aeruginosa Strain NY3. Biotech. Adv. 2010. 28(5):635-643.
Fei, X., Yin, X.; Zhang, L.; Zabriskie, T.M. Roles of VioG and VioQ in the Incorporation and Modification of the Capreomycidine Residue in the Peptide Antibiotic Viomycin. J Nat. Prod. 2007;70(4):618-622.
Yin X, Zabriskie TM. The Enduracidin Biosynthetic Gene Cluster from Streptomyces fungicidicus. Microbiology. 2006;152(10):2969-2983.
Haltli B, Tan Y, Magarvey NA, Wagenaar M, Yin X, Greenstein M, Hucul J, Zabriskie TM. Investigating β-Hydroxyenduracididine Formation in the Biosynthesis of the Mannopeptimcyins. Chemistry & Biology. 2005;12:1163-1168.
Yin X, McPhail KL, Kim K.-j, Zabriskie TM. Formation of the Nonproteinogenic Amino Acid (2S,3R)-Capreomycidine by VioD from the Viomycin Biosynthesis Pathway. ChemBioChem. 2004;5:1278-1281.
Yin X, Zabriskie TM. VioC is a Non-heme Iron, α-Ketoglutarate Dependent Oxygenase that Catalyzes the Formation of (3S)-Hydroxy-L-Arginine During Viomycin Biosynthesis. ChemBioChem. 2004;5:1274-1277.
Yin X, Proteau PJ. Characterization of Native and Histidine-tagged Deoxyxylulose 5-Phosphate Reductoisomerase from theCyanobacterium Synechocystis sp. PCC6803. Biochimica et Biophysica Acta. 2003;1652:75-81.
Cone MC, Yin X, Grochowski LL, Parker MR, Zabriskie TM. The Blasticidin S Biosynthesis Gene Cluster from Streptomyces griseochromogenes: Sequence Analysis, Organization and Initial Characterization. ChemBioChem. 2003;4:821-828.
Yin X, O'Hare T, Gould SJ, Zabriskie TM. Identification and cloning of genes encoding viomycin biosynthesis from Streptomyces vinaceus and evidence for involvement of a rare oxygenase. Gene. 2003;312:215-224.